WebThe Blue Pippin is a powerful automated tool to collect DNA fragments up to 50 kb or size select proteins. It facilitates library construction for the most popular Next Generation … WebDec 11, 2024 · The cutoff size is defined as the highest Mw band that has 10% or lower recovery. This protocol is of utility for third generation long read sequencing, where it can be used instead of the time- and sample-consuming …
E-gel vs BluePippin for narrow size selection??? - SEQanswers
WebThe system automates DNA or protein size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries, or upstream of protein mass-spec. WebAug 27, 2013 · Using Blue Pippin size selection, we have achieved yields of >500 M passed filter bases from individual SMRTcells. Below are the sequencing and assembly results of four genomes sequenced from long-insert, Blue Pippin size-selected libraries. Using only PacBio long subread data, we were able to assemble complete microbial … how many cheetahs are left in the world
iLab Organizer :: University of Auckland - Auckland Genomics
Web39 16K views 11 years ago This video demonstrates how Pippin Prep and BluePippin from Sage Science (www.sagescience.com) perform automated DNA size selection for the next-gen sequencing... WebJun 19, 2013 · This instrument allows for tight size selection of DNA samples, effectively making the peak of the size distribution much more narrow. With regard to PacBio sequencing, a narrow peak lessens the problem of preferential loading of short fragments, leading to much longer ‘reads of insert’. ... It will be nice blue pippin give us better yield ... WebThe Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection. Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases. Gel cassettes available are: 3% agarose 100-250bp isolation. 2.5% agarose 100-600bp. 1.5% agarose 250bp – 1.5Kb. how many cheetahs are brought in india