Phenyl superose
WebThere is roughly a 24,000-fold re- duction in protein, when comparing the crude extract to the Phenyl-Superose fraction 9 (Table 1). Serial 2-fold dilutions of the complementing fractions indicate that -5% of the initial SF2 is recovered in fraction 9, for … WebThe suppressive effect of the herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea on the long-term H2 photoproduction was shown to be entirely attributed to the inhibition of electron transport in PS...
Phenyl superose
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WebSep 1, 1998 · Fractions from the Bio-Scale column containing MetRS activity were pooled, were loaded onto a Phenyl-Superose column, and were eluted with a gradient of decreasing ammonium sulfate concentration. As with ion exchange chromatography, the elution profiles of MetRS activity were identical for the mitochondrial and chloroplast extracts (Fig. 1 B ). WebPolyphosphate:AMP phosphotransferase, an enzyme which catalyzes the phosphorylation of AMP to ADP at the expense of polyphosphate, was purified more than 1,500-fold from …
WebTo remove precipitated proteins, lipids, hydrophobically bound proteins or lipoproteins, refer to Appendix 1. Media characteristics Composition: sulfopropyl (SP) or quaternary amino …
WebThe cytosolic enzyme was purified to homogeneity from the moderately thermophilic archaebacterium Methanobacterium thermoautotrophicum by a combination of ammonium sulfate precipitation, four chromatographic steps (DE52, Q-Sepharose, phenyl-Superose, and Protein Pak), and native polyacrylamide gel electrophoresis. WebJul 16, 1999 · The Green A fraction was made 3 M in NaCl and injected in 1 ml aliquots into a phenyl Superose HR 5/5 FPLC column, previously equilibrated with 20 mM Tris-HCl pH 8.0, containing 1 mM DTT and 3 M NaCl. The column was washed with the same buffer and eluted with a discontinuous gradient from 3 to 0 M NaCl in 20 mM Tris-HCl pH 8.0, …
WebJan 1, 1987 · Phenyl-Superose is the most stable gel at acidic pH, whereas Phenyl-Sepharose CL-4B and Phenyl-Sepharose FF are the most stable at neutral and basic pH. INTRODUCTION One of the major areas of interest in the field of downstream processing is the use of chromatographic techniques for the large-scale isolation of biological …
WebPhenyl Sepharose ® is used in protein chromatography, affinity chromatography, hydrophobic interaction media, resins and separation media. Phenyl Sepharose ® has … short order cook ticket holderWebPhenyl-Superose Chromatography-The active fractions were diluted 10-fold with solution E (0.5 M ammonium sulfate, 10 mM imidazole-HC1 buffer, pH 7.0, 5 mM 2-mercaptoethanol) and applied to a phenyl- Superose column equilibrated with solution E. The enzyme was eluted with alinear gradient of 100% solution E to 100% solution F (5% sucrose, santa claus wall decorationsWebNov 11, 1994 · Chromatography of the proteins on the Phenyl-Superose HR5/5 column showed that the proteins with tryptophan at residue 113 have longer retention times than … short order cook slangWebPhenyl-Superose Pooled Q Sepharose fractions are diluted with an equal volume of column buffer containing 2.8 M KCl to bring the KCl concentration to 1.5 M KCl. The sample is loaded onto a 5/5 Phenyl-Superose (Pharmacia) column at a flow rate of 0.5 ml/min and washed until the UV absorbance returns to 0. short order lingoWebJul 6, 2024 · Phenyl Superose HR 10/10 column or Alkyl Superose HR 5/5 column (GE Healthcare Biosciences) was used for the separation. After the addition of ammonium sulfate to 1 m, the enzyme solution was applied to the column equilibrated with the buffer containing 1 m ammonium sulfate. santa claus village bookingWebPhenyl Sepharose CL-4B, Mono-Q 5/5 FPLC ion exchange column, Superose 6 and Superose 12 FPLC size exclusion chromatography columns, PhastGel electrophoresis gels and all molecular mass calibration ... santa claus wants someWebNov 11, 1994 · Phenyl-Superose has a negligible amount of charged groups, ensuring true hydrophobic inter- action chromatography. All the buffers and samples used in the … santa claus war bonds